Release 4.0 of the Next-Generation IEDB Tools Website is now live! This release brings all of the B cell tools to the site, including BepiPred, DiscoTope, ElliPro, and the scale-based linear epitope prediction methods.
Head over to https://nextgen-tools.iedb.org/ to try it out!
Release notes - 4.0 (Mar 18, 2026)
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New Web Tools!
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B Cell Prediction - Sequence Based: Starting from a protein sequence, this tool provides a variety of methods for predicting linear B cell epitopes. This tool includes BepiPred 3.0 as well as amino-acid scale-based methods (Chou & Fasman, Emini, Karplus & Shulz, Kolaskar & Tongaonkar, Parker) that use amino acid properties such as hydrophobicity, flexibility, and side chain protrusion to predict antigenicity.
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B Cell Prediction - Structure Based: Starting from a solved or predicted protein structure, this tool provides the Discotope 3.0 and ElliPro methods to predict antigenic regions.
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New Pipelines!
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With the introduction of the B Cell tools, new pipelines are enabled:
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B Cell - Sequence-based → T Cell Class I, T Cell Class II, Cluster, PepMatch, PepX
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B Cell - Sequence-based → T Cell Class I, T Cell Class II, Cluster, PepMatch, PepX
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PepMatch → B Cell - Sequence-based
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New/Updated Standalones!
- As a companion to the new web tools, the B Cell sequence & structure-based tools are available as command-line tools from our Downloads page.