Release 3.0 of the Next-Generation IEDB Tools Website is now live! This release brings all of the T cell class II binding, elution, and immunogenicity tools to the site as well as ICERFIRE and various other site enhancements.
Head over to https://nextgen-tools.iedb.org/ to try it out!
Release notes - 3.0 (Mar 25, 2025)
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New Web Tools!
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T cell, class II - T cell class II binding, elution, and immunogenicity predictions have been migrated from the legacy website into the Next-Generation Tools interface! This tool contains a new tab, ‘consolidated peptide table’, that collapses predictions across various alleles and methods on a per-peptide basis. Among other uses, this allows users to implement customized versions of the 7-allele peptide promiscuity method published in https://doi.org/10.1016/j.jim.2015.03.022.
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ICERFIRE - Developed in Morten Nielsen’s lab, ICERFIRE predicts neo-epitope immunogenicity by identifying the best HLA-binding ICORE. It has been integrated into the Peptide Variant Comparison tool.
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New Features!
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Interactive plots have been added to the Peptide Variant Comparison tool to allow users to quickly compare variant effects upon binding, elution, and immunogenicity.
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Additional pipelining capabilities have been incorporated, allowing users to send ‘Icore’ and ‘Core’ columns from TC1 and TC2 predictors to various tools.
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The Mutated Peptide Generator has been updated to support mouse genomes as well as multiple human genome builds.
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The Mutated Peptide Generator will now run SNPeff annotation on behalf of users, removing the requirement to provide an annotated VCF file.
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New/Updated Standalones!
- All of the command-line tools have been updated to match the capabilities of the Next-Generation Tools site. With this release, the major updates include the new T cell, class II standalone and the incorporation of ICERFIRE into the Peptide Variant Comparison standalone.